Liquid chromatography with evaporative light scattering detector
Product Introduction
The evaporative light scattering detector is used in high-performance liquid chromatography instruments to detect all samples with lower volatility than the mobile phase.
UM4800 is a universal detector that can be used in conjunction with any HPLC system. UM4800, It is the newly launched economical ELSD by Tongwei Company; It is another classic work after UM3000 and UM5000. The performance of the instrument has been further optimized, and it also has the characteristics of being economical and cost-effective. Its performance indicators have reached the international level of similar products, making it one of the better ELSD brands among similar products.
Liquid chromatography with evaporative light scattering detector
Principle Introduction
Evaporative Light Scattering Detector (ELSD) is a universal detector that detects any sample with lower volatility than the mobile phase, and the sample does not need to have light absorption characteristics.
Can solve the detection difficulties of HPLC, such as phospholipids, sugars, undifferentiated fatty acids, and amino acids, without the need for pre column and post column derivatization;
Signal response is related to sample quality and can obtain quality information of unknown substances;
Wide gradient and solvent compatibility, no solvent peak interference (multiple solvent gradients can obtain stable baselines and are less affected by temperature);
When separating complex samples, appropriate modifiers such as ammonium acetate, glacial acetic acid, formic acid can be added to the mobile phase TFA, Ammonia water, triethylamine, etc.
Application scope
ELSD has been widely used in the detection of carbohydrates, lipids, fatty acids, amino acids, drugs and polymers, especially in the determination of the effective components of Chinese medicine and traditional Chinese patent medicines and simple preparations without purple absorption.
Technical Parameter
Instrument type: Low temperature evaporation type
Temperature range of evaporation zone: room temperature to 130 ° C
Gas type: nitrogen or air
Gas flow rate range: 1-4 L/min
Baseline drift:<1.0 mV/h (1mL/min 10% methanol, evaporation temperature 40 ° C, gas flow rate 3L/min)
Minimum detection quality:<1.0 ng glucose (direct injection, 1mL/min water, evaporation temperature 40 ° C, gas flow rate 3L/min)
Typical quantitative range: 0.2-30 μ g (glucose)
Quantitative repeatability: RSD6 ≤ 2.0% (6 measurements)
Analog signal output: 0-1V
Power supply: 220V, 50HZ
Volume: 460 × 330 × 460 (mm)
Product Features
Minimum Noise Level
Ultra high sensitivity: obvious advantages of sensitivity and dynamic range
Low temperature evaporation type, can stably provide a high temperature evaporation temperature of 130 ℃
International performance index level, domestic instrument prices
Having the highest configuration among similar products: currently, the world uses a combination of semiconductor lasers and photomultiplier tubes for detection instruments
Instrument configuration
1. ELSD 1 unit
2. 1 set of workstation software
3. 1 air compressor
4. 1 set of related accessories